* . *
  • About
  • Advertise
  • Privacy & Policy
  • Contact
Monday, August 4, 2025
Earth-News
  • Home
  • Business
  • Entertainment
    Exclusive | Fox Takes Stake in IndyCar Owner Penske Entertainment – The Wall Street Journal

    Exclusive | Fox Takes Stake in IndyCar Owner Penske Entertainment – The Wall Street Journal

    Go-to entertainment: why gaming was made for the toilet – The Guardian

    Why Gaming Is the Ultimate Way to Pass Time in the Bathroom

    Chicago Youth Symphony Orchestra takes the Lollapalooza stage – Yahoo Home

    Chicago Youth Symphony Orchestra takes the Lollapalooza stage – Yahoo Home

    Sens. Blackburn, Warnock introduce CREATE Act to provide tax relief to music creators – Yahoo Home

    Sens. Blackburn and Warnock Launch CREATE Act to Deliver Tax Relief for Music Creators

    That’s (Political) Entertainment: When Theatre Meets Politics

    Future Script: How Generative AI Is Changing Collective Bargaining in the Entertainment Industry – Jackson Lewis

    Future Script: How Generative AI Is Transforming Collective Bargaining in Entertainment

  • General
  • Health
  • News

    Cracking the Code: Why China’s Economic Challenges Aren’t Shaking Markets, Unlike America’s” – Bloomberg

    Trump’s Narrow Window to Spread the Truth About Harris

    Trump’s Narrow Window to Spread the Truth About Harris

    Israel-Gaza war live updates: Hamas leader Ismail Haniyeh assassinated in Iran, group says

    Israel-Gaza war live updates: Hamas leader Ismail Haniyeh assassinated in Iran, group says

    PAP Boss to Niger Delta Youths, Stay Away from the Protest

    PAP Boss to Niger Delta Youths, Stay Away from the Protest

    Court Restricts Protests In Lagos To Freedom, Peace Park

    Court Restricts Protests In Lagos To Freedom, Peace Park

    Fans React to Jazz Jennings’ Inspiring Weight Loss Journey

    Fans React to Jazz Jennings’ Inspiring Weight Loss Journey

    Trending Tags

    • Trump Inauguration
    • United Stated
    • White House
    • Market Stories
    • Election Results
  • Science
  • Sports
  • Technology
    Credo Technology: Wiring The AI Revolution (NASDAQ:CRDO) – Seeking Alpha

    Credo Technology: Driving the Next Wave of AI Innovation

    Microsoft Seeks to Extend Access to OpenAI Technology – PYMNTS.com

    Microsoft Aims to Broaden Access to OpenAI Technology

    Livonia police use grappler technology to stop drunk driver – ClickOnDetroit | WDIV Local 4

    Livonia Police Deploy Grappler Technology to Safely Stop Drunk Driver

    Emory orthopaedic surgeons use robotic technology to transform knee replacement surgery – Emory News Center

    How Robotic Technology is Revolutionizing Knee Replacement Surgery

    Cognizant Technology Solutions Corp (CTSH) Q2 2025 Earnings Call Highlights: Strong Revenue … – Yahoo.co

    Cognizant Q2 2025 Earnings: Impressive Revenue Growth and Key Takeaways

    Revving Up The U.S. Technology Engine – Forbes

    Revving Up The U.S. Technology Engine – Forbes

    Trending Tags

    • Nintendo Switch
    • CES 2017
    • Playstation 4 Pro
    • Mark Zuckerberg
No Result
View All Result
  • Home
  • Business
  • Entertainment
    Exclusive | Fox Takes Stake in IndyCar Owner Penske Entertainment – The Wall Street Journal

    Exclusive | Fox Takes Stake in IndyCar Owner Penske Entertainment – The Wall Street Journal

    Go-to entertainment: why gaming was made for the toilet – The Guardian

    Why Gaming Is the Ultimate Way to Pass Time in the Bathroom

    Chicago Youth Symphony Orchestra takes the Lollapalooza stage – Yahoo Home

    Chicago Youth Symphony Orchestra takes the Lollapalooza stage – Yahoo Home

    Sens. Blackburn, Warnock introduce CREATE Act to provide tax relief to music creators – Yahoo Home

    Sens. Blackburn and Warnock Launch CREATE Act to Deliver Tax Relief for Music Creators

    That’s (Political) Entertainment: When Theatre Meets Politics

    Future Script: How Generative AI Is Changing Collective Bargaining in the Entertainment Industry – Jackson Lewis

    Future Script: How Generative AI Is Transforming Collective Bargaining in Entertainment

  • General
  • Health
  • News

    Cracking the Code: Why China’s Economic Challenges Aren’t Shaking Markets, Unlike America’s” – Bloomberg

    Trump’s Narrow Window to Spread the Truth About Harris

    Trump’s Narrow Window to Spread the Truth About Harris

    Israel-Gaza war live updates: Hamas leader Ismail Haniyeh assassinated in Iran, group says

    Israel-Gaza war live updates: Hamas leader Ismail Haniyeh assassinated in Iran, group says

    PAP Boss to Niger Delta Youths, Stay Away from the Protest

    PAP Boss to Niger Delta Youths, Stay Away from the Protest

    Court Restricts Protests In Lagos To Freedom, Peace Park

    Court Restricts Protests In Lagos To Freedom, Peace Park

    Fans React to Jazz Jennings’ Inspiring Weight Loss Journey

    Fans React to Jazz Jennings’ Inspiring Weight Loss Journey

    Trending Tags

    • Trump Inauguration
    • United Stated
    • White House
    • Market Stories
    • Election Results
  • Science
  • Sports
  • Technology
    Credo Technology: Wiring The AI Revolution (NASDAQ:CRDO) – Seeking Alpha

    Credo Technology: Driving the Next Wave of AI Innovation

    Microsoft Seeks to Extend Access to OpenAI Technology – PYMNTS.com

    Microsoft Aims to Broaden Access to OpenAI Technology

    Livonia police use grappler technology to stop drunk driver – ClickOnDetroit | WDIV Local 4

    Livonia Police Deploy Grappler Technology to Safely Stop Drunk Driver

    Emory orthopaedic surgeons use robotic technology to transform knee replacement surgery – Emory News Center

    How Robotic Technology is Revolutionizing Knee Replacement Surgery

    Cognizant Technology Solutions Corp (CTSH) Q2 2025 Earnings Call Highlights: Strong Revenue … – Yahoo.co

    Cognizant Q2 2025 Earnings: Impressive Revenue Growth and Key Takeaways

    Revving Up The U.S. Technology Engine – Forbes

    Revving Up The U.S. Technology Engine – Forbes

    Trending Tags

    • Nintendo Switch
    • CES 2017
    • Playstation 4 Pro
    • Mark Zuckerberg
No Result
View All Result
Earth-News
No Result
View All Result
Home Science

A Breakthrough in Genome Editing: How NICER Outperforms CRISPR/Cas9

December 15, 2023
in Science
A Breakthrough in Genome Editing: How NICER Outperforms CRISPR/Cas9
Share on FacebookShare on Twitter

Genetic Editing DNA Concept

NICER, a new gene editing method developed by Japanese researchers, presents a safer alternative to CRISPR/Cas9 by significantly reducing unintended DNA mutations, enhancing genetic disease treatment.

Researchers led by Osaka University develop a new gene modification technique known as NICER that significantly reduces off-target mutations in DNA.

The gene editing technique CRISPR/Cas9 has allowed researchers to make precise and impactful changes to an organism’s DNA to fix mutations that cause genetic disease. However, the CRISPR/Cas9 method can also result in unintended DNA mutations that may have negative effects. Recently, researchers in Japan have developed a new gene editing technique that is as effective as CRISPR/Cas9 while significantly reducing these unintended mutations.

Off-Target Mutations Resulting From Conventional Genome Editing Methods

Off-target mutations resulting from conventional genome editing methods. Due to the mutagenic nature of DNA double-strand break repair, even if the gene mutation is successfully corrected, there is a heightened risk of off-target mutations in areas distinct from the original target of CRISPR/Cas9. Credit: S. Nakada

Understanding NICER’s Methodology

In a new study published in the journal Nature Communications, researchers led by Osaka University introduced a novel technique called NICER, which is based on the creation of multiple small cuts in single DNA strands by an enzyme called a nickase.

Traditional CRISPR/Cas9 editing uses small pieces of genetic code called guide RNAs and an enzyme called Cas9. The guide RNAs target a specific section of the DNA and the Cas9 enzyme initiates a break in the double-stranded DNA structure at this location. This double-strand break is key for initiating changes to the DNA. However, cellular repair of double-strand breaks can lead to unintended DNA mutations, as well as the integration of exogenous DNA to the human genome, which raises safety concerns for clinical applications of CRISPR/Cas9 technology. To minimize these unintended mutations, the Osaka University-led research team investigated the use of Cas9 nickase, which creates single-strand breaks or “nicks” in DNA that are typically repaired without causing mutations.

NICER’s Advantages and Applications

Genome Editing Using the Cas9 Mutant Nickase

Genome editing using the Cas9 mutant, nickase. Nickase induces single-strand breaks (nicks) instead of DNA double-strand breaks. Nicks are typically repaired without causing mutations. Hence, even if nicks appear at sites different from nickase’s original target, new gene mutations are seldom seen at these locations. This approach allows for precise gene correction with minimal off-target mutations. Credit: S. Nakada

“Each chromosome in the genome has a ‘homologous’ copy,” says lead author of the study Akiko Tomita. “Using the NICER technique, heterozygous mutations – in which a mutation appears in one chromosome but not its homologous copy – are repaired using the unmutated homologous chromosome as a template.”

For their initial experiments, the research team used human lymphoblast cells with a known heterozygous mutation in a gene called TK1. When these cells were treated with nickase to induce a single cut in the TK1 region, TK1 activity was recovered at a low rate. However, when the nickase induced multiple nicks in this region on both homologous chromosomes, gene correction efficiency was enhanced approximately seventeen-fold via activation of a cellular repair mechanism.

NICER Gene Editing Method Schematic

Schematic of the NICER gene editing method. A nick is made near the mutation (and is exclusive to the mutant allele). Merely introducing a nick close to the mutation instigates homologous recombination between homologous chromosomes but at a very low rate. However, when one or two additional nicks are made to both alleles, the gene correction efficiency through interhomolog homologous recombination sees a significant boost. Credit: S. Nakada

“Further genomic analysis showed that the NICER technique rarely induced off-target mutations,” says senior author Shinichiro Nakada. “We were also pleased to find that NICER was able to restore the expression of disease-causing genes in cells derived from genetic diseases involving compound heterozygous mutations.”

Because the NICER method does not involve DNA double-strand breaks or the use of exogenous DNA, this technique appears to be a safe alternative to conventional CRISPR/Cas9 methods. NICER may represent a novel approach for the treatment of genetic diseases caused by heterozygous mutations.

Reference: “Inducing multiple nicks promotes interhomolog homologous recombination to correct heterozygous mutations in somatic cells” by Akiko Tomita, Hiroyuki Sasanuma, Tomoo Owa, Yuka Nakazawa, Mayuko Shimada, Takahiro Fukuoka, Tomoo Ogi and Shinichiro Nakada, 15 September 2023, Nature Communications.
DOI: 10.1038/s41467-023-41048-5

>>> Read full article>>>
Copyright for syndicated content belongs to the linked Source : SciTechDaily – https://scitechdaily.com/a-breakthrough-in-genome-editing-how-nicer-outperforms-crispr-cas9/

Tags: BreakthroughGenomescience
Previous Post

Sanwo-Olu to provide health palliatives for residents, subsidise prices of medication

Next Post

A Neuroscience Milestone: Complete Cellular Mapping of a Mammalian Brain

New rule would expand tug escort requirements, reduce risk of oil spills in Puget Sound – Washington State Department of Ecology (.gov)

New Rule Strengthens Tug Escort Requirements to Drastically Reduce Oil Spill Risks in Puget Sound

August 4, 2025
100 years ago, scientists thought we’d be eating food made from air – Popular Science

A Century Ago, Scientists Predicted We’d Be Eating Food Made from Air

August 4, 2025
Maserati and Sparco Launch Lifestyle Capsule Inspired by Motorsport Heritage – stupidDOPE

Maserati and Sparco Launch Lifestyle Capsule Inspired by Motorsport Heritage – stupidDOPE

August 4, 2025
Fox News Flash top entertainment headlines of the week – Fox News

Top Entertainment Headlines You Can’t Miss This Week

August 4, 2025
How Is the Economy Doing Right Now? – NerdWallet

What’s Really Going On with the Economy Right Now?

August 4, 2025
Exclusive | Fox Takes Stake in IndyCar Owner Penske Entertainment – The Wall Street Journal

Exclusive | Fox Takes Stake in IndyCar Owner Penske Entertainment – The Wall Street Journal

August 4, 2025
At Washington Health Care Authority, workers are warned of layoffs – Washington State Standard

Washington Health Care Authority Alerts Employees of Potential Layoffs

August 4, 2025
NYPD program allowed slain officer to moonlight as private security guard – Spectrum News NY1

NYPD program allowed slain officer to moonlight as private security guard – Spectrum News NY1

August 4, 2025
Credo Technology: Wiring The AI Revolution (NASDAQ:CRDO) – Seeking Alpha

Credo Technology: Driving the Next Wave of AI Innovation

August 3, 2025
Judge Halts Stephen F. Austin’s Female Sports Cuts Amid Title IX Suit – Sportico.com

Judge Halts Stephen F. Austin’s Female Sports Cuts Amid Title IX Suit – Sportico.com

August 3, 2025

Categories

Archives

August 2025
MTWTFSS
 123
45678910
11121314151617
18192021222324
25262728293031
« Jul    
Earth-News.info

The Earth News is an independent English-language daily published Website from all around the World News

Browse by Category

  • Business (20,132)
  • Ecology (753)
  • Economy (778)
  • Entertainment (21,655)
  • General (16,273)
  • Health (9,815)
  • Lifestyle (786)
  • News (22,149)
  • People (777)
  • Politics (787)
  • Science (15,991)
  • Sports (21,273)
  • Technology (15,755)
  • World (759)

Recent News

New rule would expand tug escort requirements, reduce risk of oil spills in Puget Sound – Washington State Department of Ecology (.gov)

New Rule Strengthens Tug Escort Requirements to Drastically Reduce Oil Spill Risks in Puget Sound

August 4, 2025
100 years ago, scientists thought we’d be eating food made from air – Popular Science

A Century Ago, Scientists Predicted We’d Be Eating Food Made from Air

August 4, 2025
  • About
  • Advertise
  • Privacy & Policy
  • Contact

© 2023 earth-news.info

No Result
View All Result

© 2023 earth-news.info

No Result
View All Result

© 2023 earth-news.info

Go to mobile version