* . *
  • About
  • Advertise
  • Privacy & Policy
  • Contact
Sunday, October 5, 2025
Earth-News
  • Home
  • Business
  • Entertainment
    Why Taylor Swift Name-Dropped Elizabeth Taylor in Her New Album – Yahoo

    Here’s Why Taylor Swift Dropped Elizabeth Taylor’s Name in Her New Album

    Al Roker Gives Olivia Dean an Unexpected ‘New Job’ on the ‘Today’ Show – Yahoo

    Al Roker Shocks Olivia Dean with an Exciting New Role on the ‘Today’ Show

    Books about the arts and some haunts for a Denton October – Denton Record-Chronicle

    Uncover Artistic Treasures and Spooky Adventures to Experience in Denton This October

    Taylor Swift Releases New Album The Life of a Showgirl : Listen and Read the Full Credits – Yahoo

    Taylor Swift Releases New Album The Life of a Showgirl : Listen and Read the Full Credits – Yahoo

    Toni Braxton Is Turning Her Biggest Hits Into Lifetime Movies – Yahoo

    Toni Braxton Is Turning Her Biggest Hits Into Lifetime Movies – Yahoo

    Major airline to offer new in-flight entertainment options for passengers – PennLive.com

    Major airline to offer new in-flight entertainment options for passengers – PennLive.com

  • General
  • Health
  • News

    Cracking the Code: Why China’s Economic Challenges Aren’t Shaking Markets, Unlike America’s” – Bloomberg

    Trump’s Narrow Window to Spread the Truth About Harris

    Trump’s Narrow Window to Spread the Truth About Harris

    Israel-Gaza war live updates: Hamas leader Ismail Haniyeh assassinated in Iran, group says

    Israel-Gaza war live updates: Hamas leader Ismail Haniyeh assassinated in Iran, group says

    PAP Boss to Niger Delta Youths, Stay Away from the Protest

    PAP Boss to Niger Delta Youths, Stay Away from the Protest

    Court Restricts Protests In Lagos To Freedom, Peace Park

    Court Restricts Protests In Lagos To Freedom, Peace Park

    Fans React to Jazz Jennings’ Inspiring Weight Loss Journey

    Fans React to Jazz Jennings’ Inspiring Weight Loss Journey

    Trending Tags

    • Trump Inauguration
    • United Stated
    • White House
    • Market Stories
    • Election Results
  • Science
  • Sports
  • Technology
    What the Recent Price Surge Means for Figure Technology Solutions After SEC Settlement – Yahoo Finance

    What the Recent Price Surge Reveals About Figure Technology Solutions Following SEC Settlement

    MAC Brings iPad Technology to Football Sidelines Across All 13 Member Schools – Sports Video Group

    MAC Brings iPad Technology to Football Sidelines Across All 13 Member Schools – Sports Video Group

    Technology Is Becoming More Important Than Humans In CX – No Jitter

    Technology Is Becoming More Important Than Humans In CX – No Jitter

    A Tech Expo Shows What China Can Make, but Not Who’ll Buy It All – The New York Times

    Inside China’s Tech Expo: Cutting-Edge Innovations Face Uncertain Demand

    Steampunk Metal Oval Technology Sense Sunglasses Personality Handmade Chain Multicolor Sunglasses UV400 – The San Joaquin Valley Sun

    Steampunk Metal Oval Sunglasses with Handmade Multicolor Chain – Bold UV400 Protection and Unique Style

    STELLA Automotive AI Appoints Fred Seidelman as Chief Technology Officer – Yahoo Finance

    STELLA Automotive AI Appoints Fred Seidelman as New Chief Technology Officer

    Trending Tags

    • Nintendo Switch
    • CES 2017
    • Playstation 4 Pro
    • Mark Zuckerberg
No Result
View All Result
  • Home
  • Business
  • Entertainment
    Why Taylor Swift Name-Dropped Elizabeth Taylor in Her New Album – Yahoo

    Here’s Why Taylor Swift Dropped Elizabeth Taylor’s Name in Her New Album

    Al Roker Gives Olivia Dean an Unexpected ‘New Job’ on the ‘Today’ Show – Yahoo

    Al Roker Shocks Olivia Dean with an Exciting New Role on the ‘Today’ Show

    Books about the arts and some haunts for a Denton October – Denton Record-Chronicle

    Uncover Artistic Treasures and Spooky Adventures to Experience in Denton This October

    Taylor Swift Releases New Album The Life of a Showgirl : Listen and Read the Full Credits – Yahoo

    Taylor Swift Releases New Album The Life of a Showgirl : Listen and Read the Full Credits – Yahoo

    Toni Braxton Is Turning Her Biggest Hits Into Lifetime Movies – Yahoo

    Toni Braxton Is Turning Her Biggest Hits Into Lifetime Movies – Yahoo

    Major airline to offer new in-flight entertainment options for passengers – PennLive.com

    Major airline to offer new in-flight entertainment options for passengers – PennLive.com

  • General
  • Health
  • News

    Cracking the Code: Why China’s Economic Challenges Aren’t Shaking Markets, Unlike America’s” – Bloomberg

    Trump’s Narrow Window to Spread the Truth About Harris

    Trump’s Narrow Window to Spread the Truth About Harris

    Israel-Gaza war live updates: Hamas leader Ismail Haniyeh assassinated in Iran, group says

    Israel-Gaza war live updates: Hamas leader Ismail Haniyeh assassinated in Iran, group says

    PAP Boss to Niger Delta Youths, Stay Away from the Protest

    PAP Boss to Niger Delta Youths, Stay Away from the Protest

    Court Restricts Protests In Lagos To Freedom, Peace Park

    Court Restricts Protests In Lagos To Freedom, Peace Park

    Fans React to Jazz Jennings’ Inspiring Weight Loss Journey

    Fans React to Jazz Jennings’ Inspiring Weight Loss Journey

    Trending Tags

    • Trump Inauguration
    • United Stated
    • White House
    • Market Stories
    • Election Results
  • Science
  • Sports
  • Technology
    What the Recent Price Surge Means for Figure Technology Solutions After SEC Settlement – Yahoo Finance

    What the Recent Price Surge Reveals About Figure Technology Solutions Following SEC Settlement

    MAC Brings iPad Technology to Football Sidelines Across All 13 Member Schools – Sports Video Group

    MAC Brings iPad Technology to Football Sidelines Across All 13 Member Schools – Sports Video Group

    Technology Is Becoming More Important Than Humans In CX – No Jitter

    Technology Is Becoming More Important Than Humans In CX – No Jitter

    A Tech Expo Shows What China Can Make, but Not Who’ll Buy It All – The New York Times

    Inside China’s Tech Expo: Cutting-Edge Innovations Face Uncertain Demand

    Steampunk Metal Oval Technology Sense Sunglasses Personality Handmade Chain Multicolor Sunglasses UV400 – The San Joaquin Valley Sun

    Steampunk Metal Oval Sunglasses with Handmade Multicolor Chain – Bold UV400 Protection and Unique Style

    STELLA Automotive AI Appoints Fred Seidelman as Chief Technology Officer – Yahoo Finance

    STELLA Automotive AI Appoints Fred Seidelman as New Chief Technology Officer

    Trending Tags

    • Nintendo Switch
    • CES 2017
    • Playstation 4 Pro
    • Mark Zuckerberg
No Result
View All Result
Earth-News
No Result
View All Result
Home Science

A Breakthrough in Genome Editing: How NICER Outperforms CRISPR/Cas9

December 15, 2023
in Science
A Breakthrough in Genome Editing: How NICER Outperforms CRISPR/Cas9
Share on FacebookShare on Twitter

Genetic Editing DNA Concept

NICER, a new gene editing method developed by Japanese researchers, presents a safer alternative to CRISPR/Cas9 by significantly reducing unintended DNA mutations, enhancing genetic disease treatment.

Researchers led by Osaka University develop a new gene modification technique known as NICER that significantly reduces off-target mutations in DNA.

The gene editing technique CRISPR/Cas9 has allowed researchers to make precise and impactful changes to an organism’s DNA to fix mutations that cause genetic disease. However, the CRISPR/Cas9 method can also result in unintended DNA mutations that may have negative effects. Recently, researchers in Japan have developed a new gene editing technique that is as effective as CRISPR/Cas9 while significantly reducing these unintended mutations.

Off-Target Mutations Resulting From Conventional Genome Editing Methods

Off-target mutations resulting from conventional genome editing methods. Due to the mutagenic nature of DNA double-strand break repair, even if the gene mutation is successfully corrected, there is a heightened risk of off-target mutations in areas distinct from the original target of CRISPR/Cas9. Credit: S. Nakada

Understanding NICER’s Methodology

In a new study published in the journal Nature Communications, researchers led by Osaka University introduced a novel technique called NICER, which is based on the creation of multiple small cuts in single DNA strands by an enzyme called a nickase.

Traditional CRISPR/Cas9 editing uses small pieces of genetic code called guide RNAs and an enzyme called Cas9. The guide RNAs target a specific section of the DNA and the Cas9 enzyme initiates a break in the double-stranded DNA structure at this location. This double-strand break is key for initiating changes to the DNA. However, cellular repair of double-strand breaks can lead to unintended DNA mutations, as well as the integration of exogenous DNA to the human genome, which raises safety concerns for clinical applications of CRISPR/Cas9 technology. To minimize these unintended mutations, the Osaka University-led research team investigated the use of Cas9 nickase, which creates single-strand breaks or “nicks” in DNA that are typically repaired without causing mutations.

NICER’s Advantages and Applications

Genome Editing Using the Cas9 Mutant Nickase

Genome editing using the Cas9 mutant, nickase. Nickase induces single-strand breaks (nicks) instead of DNA double-strand breaks. Nicks are typically repaired without causing mutations. Hence, even if nicks appear at sites different from nickase’s original target, new gene mutations are seldom seen at these locations. This approach allows for precise gene correction with minimal off-target mutations. Credit: S. Nakada

“Each chromosome in the genome has a ‘homologous’ copy,” says lead author of the study Akiko Tomita. “Using the NICER technique, heterozygous mutations – in which a mutation appears in one chromosome but not its homologous copy – are repaired using the unmutated homologous chromosome as a template.”

For their initial experiments, the research team used human lymphoblast cells with a known heterozygous mutation in a gene called TK1. When these cells were treated with nickase to induce a single cut in the TK1 region, TK1 activity was recovered at a low rate. However, when the nickase induced multiple nicks in this region on both homologous chromosomes, gene correction efficiency was enhanced approximately seventeen-fold via activation of a cellular repair mechanism.

NICER Gene Editing Method Schematic

Schematic of the NICER gene editing method. A nick is made near the mutation (and is exclusive to the mutant allele). Merely introducing a nick close to the mutation instigates homologous recombination between homologous chromosomes but at a very low rate. However, when one or two additional nicks are made to both alleles, the gene correction efficiency through interhomolog homologous recombination sees a significant boost. Credit: S. Nakada

“Further genomic analysis showed that the NICER technique rarely induced off-target mutations,” says senior author Shinichiro Nakada. “We were also pleased to find that NICER was able to restore the expression of disease-causing genes in cells derived from genetic diseases involving compound heterozygous mutations.”

Because the NICER method does not involve DNA double-strand breaks or the use of exogenous DNA, this technique appears to be a safe alternative to conventional CRISPR/Cas9 methods. NICER may represent a novel approach for the treatment of genetic diseases caused by heterozygous mutations.

Reference: “Inducing multiple nicks promotes interhomolog homologous recombination to correct heterozygous mutations in somatic cells” by Akiko Tomita, Hiroyuki Sasanuma, Tomoo Owa, Yuka Nakazawa, Mayuko Shimada, Takahiro Fukuoka, Tomoo Ogi and Shinichiro Nakada, 15 September 2023, Nature Communications.
DOI: 10.1038/s41467-023-41048-5

>>> Read full article>>>
Copyright for syndicated content belongs to the linked Source : SciTechDaily – https://scitechdaily.com/a-breakthrough-in-genome-editing-how-nicer-outperforms-crispr-cas9/

Tags: BreakthroughGenomescience
Previous Post

Sanwo-Olu to provide health palliatives for residents, subsidise prices of medication

Next Post

A Neuroscience Milestone: Complete Cellular Mapping of a Mammalian Brain

First Record of Mating Involving a Melanistic Jaguar (Panthera onca) in the Wild: Novel Behavioural Insights Into Colour Morphs and Captive‐Wild Comparisons – Luypaert – 2025 – Ecology and Evolution – Wiley Online Library

First Record of Mating Involving a Melanistic Jaguar (Panthera onca) in the Wild: Novel Behavioural Insights Into Colour Morphs and Captive‐Wild Comparisons – Luypaert – 2025 – Ecology and Evolution – Wiley Online Library

October 5, 2025
Multi-generational vulture nests hold 700 years of human artifacts – Popular Science

Multi-generational vulture nests hold 700 years of human artifacts – Popular Science

October 5, 2025
Chinese tech company develops creepy ultra-lifelike robot face — watch it blink, twitch and nod – Live Science

Chinese tech company develops creepy ultra-lifelike robot face — watch it blink, twitch and nod – Live Science

October 5, 2025
FSE Lifestyle Services (HKG:331) Has Announced That Its Dividend Will Be Reduced To HK$0.185 – simplywall.st

FSE Lifestyle Services (HKG:331) Has Announced That Its Dividend Will Be Reduced To HK$0.185 – simplywall.st

October 5, 2025
Irrigation technology adapts to water restrictions – Farm Progress

Innovative Irrigation Technologies Transform Farming Amid Water Restrictions

October 5, 2025
Allende, Alba and Messi help Miami beat Revolution 4-1 – Yahoo Sports

Allende, Alba, and Messi Ignite Miami’s Stunning 4-1 Triumph Over Revolution

October 5, 2025
Watch the October 2025 Edition of the World Report – newsroom.churchofjesuschrist.org

Watch the October 2025 Edition of the World Report – newsroom.churchofjesuschrist.org

October 5, 2025
Shutdowns usually don’t do much economic damage, but there are reasons to worry this time – PBS

Why This Shutdown Could Hit the Economy Harder Than Usual

October 5, 2025
Why Taylor Swift Name-Dropped Elizabeth Taylor in Her New Album – Yahoo

Here’s Why Taylor Swift Dropped Elizabeth Taylor’s Name in Her New Album

October 5, 2025

Unleash Your Potential: Join the Franciscan Health Women’s Conference 2025!

October 5, 2025

Categories

Archives

October 2025
M T W T F S S
 12345
6789101112
13141516171819
20212223242526
2728293031  
« Sep    
Earth-News.info

The Earth News is an independent English-language daily published Website from all around the World News

Browse by Category

  • Business (20,132)
  • Ecology (852)
  • Economy (872)
  • Entertainment (21,746)
  • General (17,419)
  • Health (9,914)
  • Lifestyle (885)
  • News (22,149)
  • People (874)
  • Politics (883)
  • Science (16,083)
  • Sports (21,373)
  • Technology (15,854)
  • World (855)

Recent News

First Record of Mating Involving a Melanistic Jaguar (Panthera onca) in the Wild: Novel Behavioural Insights Into Colour Morphs and Captive‐Wild Comparisons – Luypaert – 2025 – Ecology and Evolution – Wiley Online Library

First Record of Mating Involving a Melanistic Jaguar (Panthera onca) in the Wild: Novel Behavioural Insights Into Colour Morphs and Captive‐Wild Comparisons – Luypaert – 2025 – Ecology and Evolution – Wiley Online Library

October 5, 2025
Multi-generational vulture nests hold 700 years of human artifacts – Popular Science

Multi-generational vulture nests hold 700 years of human artifacts – Popular Science

October 5, 2025
  • About
  • Advertise
  • Privacy & Policy
  • Contact

© 2023 earth-news.info

No Result
View All Result

© 2023 earth-news.info

No Result
View All Result

© 2023 earth-news.info

Go to mobile version