* . *
  • About
  • Advertise
  • Privacy & Policy
  • Contact
Thursday, October 23, 2025
Earth-News
  • Home
  • Business
  • Entertainment
    Reba McEntire Details Personal Relationship With Late Stepson Brandon Blackstock – KNDU

    Reba McEntire Shares Emotional Tribute to Her Late Stepson Brandon Blackstock

    Sacramento city leaders approve adding 2 entertainment zones in midtown – CBS News

    Sacramento City Leaders Approve Two Thrilling New Entertainment Zones in Midtown

    AMC brings first new Dolby Experience to Gwinnett since 2017 – Wyoming News Now

    AMC Launches First New Dolby Experience in Gwinnett Since 2017

    Hetzel Design: blending architecture and entertainment – Blooloop

    Hetzel Design: Where Architecture and Entertainment Unite in Perfect Harmony

    Country music legend rushed to hospital year after heart surgery. Here’s what we know – PennLive.com

    Country Music Legend Rushed to Hospital One Year After Heart Surgery – What’s Happening Now?

    Strictly Come Dancing results: Chris Robshaw is eliminated while drag queen La Voix escapes dance-off – Yahoo

    Strictly Come Dancing results: Chris Robshaw is eliminated while drag queen La Voix escapes dance-off – Yahoo

  • General
  • Health
  • News

    Cracking the Code: Why China’s Economic Challenges Aren’t Shaking Markets, Unlike America’s” – Bloomberg

    Trump’s Narrow Window to Spread the Truth About Harris

    Trump’s Narrow Window to Spread the Truth About Harris

    Israel-Gaza war live updates: Hamas leader Ismail Haniyeh assassinated in Iran, group says

    Israel-Gaza war live updates: Hamas leader Ismail Haniyeh assassinated in Iran, group says

    PAP Boss to Niger Delta Youths, Stay Away from the Protest

    PAP Boss to Niger Delta Youths, Stay Away from the Protest

    Court Restricts Protests In Lagos To Freedom, Peace Park

    Court Restricts Protests In Lagos To Freedom, Peace Park

    Fans React to Jazz Jennings’ Inspiring Weight Loss Journey

    Fans React to Jazz Jennings’ Inspiring Weight Loss Journey

    Trending Tags

    • Trump Inauguration
    • United Stated
    • White House
    • Market Stories
    • Election Results
  • Science
  • Sports
  • Technology
    Next steps: Technology opens new options for greater mobility – Missouri Independent

    Next Steps: How Technology is Opening Exciting New Doors to Greater Mobility

    Rydberg Technologies Inc. Announces Launch of Rydberg Photonics in Berlin – The Quantum Insider

    Rydberg Technologies Launches Exciting New Photonics Division in Berlin

    A look into new technology at Columbia University that could help prevent a dangerous pregnancy complication – ABC7 New York

    A look into new technology at Columbia University that could help prevent a dangerous pregnancy complication – ABC7 New York

    Office Technology: Dealers’ Managed IT Revenue up Nearly 30% – The Cannata Report –

    Office Technology: Dealers’ Managed IT Revenue up Nearly 30% – The Cannata Report –

    3 E Network Technology Group Limited Closes $1.5 Million Convertible Promissory Note Offering – Quiver Quantitative

    3 E Network Technology Group Limited Closes $1.5 Million Convertible Promissory Note Offering – Quiver Quantitative

    3 Technology Stocks to Buy Now – Yahoo Finance

    3 Must-Buy Tech Stocks You Can’t Afford to Miss Right Now

    Trending Tags

    • Nintendo Switch
    • CES 2017
    • Playstation 4 Pro
    • Mark Zuckerberg
No Result
View All Result
  • Home
  • Business
  • Entertainment
    Reba McEntire Details Personal Relationship With Late Stepson Brandon Blackstock – KNDU

    Reba McEntire Shares Emotional Tribute to Her Late Stepson Brandon Blackstock

    Sacramento city leaders approve adding 2 entertainment zones in midtown – CBS News

    Sacramento City Leaders Approve Two Thrilling New Entertainment Zones in Midtown

    AMC brings first new Dolby Experience to Gwinnett since 2017 – Wyoming News Now

    AMC Launches First New Dolby Experience in Gwinnett Since 2017

    Hetzel Design: blending architecture and entertainment – Blooloop

    Hetzel Design: Where Architecture and Entertainment Unite in Perfect Harmony

    Country music legend rushed to hospital year after heart surgery. Here’s what we know – PennLive.com

    Country Music Legend Rushed to Hospital One Year After Heart Surgery – What’s Happening Now?

    Strictly Come Dancing results: Chris Robshaw is eliminated while drag queen La Voix escapes dance-off – Yahoo

    Strictly Come Dancing results: Chris Robshaw is eliminated while drag queen La Voix escapes dance-off – Yahoo

  • General
  • Health
  • News

    Cracking the Code: Why China’s Economic Challenges Aren’t Shaking Markets, Unlike America’s” – Bloomberg

    Trump’s Narrow Window to Spread the Truth About Harris

    Trump’s Narrow Window to Spread the Truth About Harris

    Israel-Gaza war live updates: Hamas leader Ismail Haniyeh assassinated in Iran, group says

    Israel-Gaza war live updates: Hamas leader Ismail Haniyeh assassinated in Iran, group says

    PAP Boss to Niger Delta Youths, Stay Away from the Protest

    PAP Boss to Niger Delta Youths, Stay Away from the Protest

    Court Restricts Protests In Lagos To Freedom, Peace Park

    Court Restricts Protests In Lagos To Freedom, Peace Park

    Fans React to Jazz Jennings’ Inspiring Weight Loss Journey

    Fans React to Jazz Jennings’ Inspiring Weight Loss Journey

    Trending Tags

    • Trump Inauguration
    • United Stated
    • White House
    • Market Stories
    • Election Results
  • Science
  • Sports
  • Technology
    Next steps: Technology opens new options for greater mobility – Missouri Independent

    Next Steps: How Technology is Opening Exciting New Doors to Greater Mobility

    Rydberg Technologies Inc. Announces Launch of Rydberg Photonics in Berlin – The Quantum Insider

    Rydberg Technologies Launches Exciting New Photonics Division in Berlin

    A look into new technology at Columbia University that could help prevent a dangerous pregnancy complication – ABC7 New York

    A look into new technology at Columbia University that could help prevent a dangerous pregnancy complication – ABC7 New York

    Office Technology: Dealers’ Managed IT Revenue up Nearly 30% – The Cannata Report –

    Office Technology: Dealers’ Managed IT Revenue up Nearly 30% – The Cannata Report –

    3 E Network Technology Group Limited Closes $1.5 Million Convertible Promissory Note Offering – Quiver Quantitative

    3 E Network Technology Group Limited Closes $1.5 Million Convertible Promissory Note Offering – Quiver Quantitative

    3 Technology Stocks to Buy Now – Yahoo Finance

    3 Must-Buy Tech Stocks You Can’t Afford to Miss Right Now

    Trending Tags

    • Nintendo Switch
    • CES 2017
    • Playstation 4 Pro
    • Mark Zuckerberg
No Result
View All Result
Earth-News
No Result
View All Result
Home Science

A Breakthrough in Genome Editing: How NICER Outperforms CRISPR/Cas9

December 15, 2023
in Science
A Breakthrough in Genome Editing: How NICER Outperforms CRISPR/Cas9
Share on FacebookShare on Twitter

Genetic Editing DNA Concept

NICER, a new gene editing method developed by Japanese researchers, presents a safer alternative to CRISPR/Cas9 by significantly reducing unintended DNA mutations, enhancing genetic disease treatment.

Researchers led by Osaka University develop a new gene modification technique known as NICER that significantly reduces off-target mutations in DNA.

The gene editing technique CRISPR/Cas9 has allowed researchers to make precise and impactful changes to an organism’s DNA to fix mutations that cause genetic disease. However, the CRISPR/Cas9 method can also result in unintended DNA mutations that may have negative effects. Recently, researchers in Japan have developed a new gene editing technique that is as effective as CRISPR/Cas9 while significantly reducing these unintended mutations.

Off-Target Mutations Resulting From Conventional Genome Editing Methods

Off-target mutations resulting from conventional genome editing methods. Due to the mutagenic nature of DNA double-strand break repair, even if the gene mutation is successfully corrected, there is a heightened risk of off-target mutations in areas distinct from the original target of CRISPR/Cas9. Credit: S. Nakada

Understanding NICER’s Methodology

In a new study published in the journal Nature Communications, researchers led by Osaka University introduced a novel technique called NICER, which is based on the creation of multiple small cuts in single DNA strands by an enzyme called a nickase.

Traditional CRISPR/Cas9 editing uses small pieces of genetic code called guide RNAs and an enzyme called Cas9. The guide RNAs target a specific section of the DNA and the Cas9 enzyme initiates a break in the double-stranded DNA structure at this location. This double-strand break is key for initiating changes to the DNA. However, cellular repair of double-strand breaks can lead to unintended DNA mutations, as well as the integration of exogenous DNA to the human genome, which raises safety concerns for clinical applications of CRISPR/Cas9 technology. To minimize these unintended mutations, the Osaka University-led research team investigated the use of Cas9 nickase, which creates single-strand breaks or “nicks” in DNA that are typically repaired without causing mutations.

NICER’s Advantages and Applications

Genome Editing Using the Cas9 Mutant Nickase

Genome editing using the Cas9 mutant, nickase. Nickase induces single-strand breaks (nicks) instead of DNA double-strand breaks. Nicks are typically repaired without causing mutations. Hence, even if nicks appear at sites different from nickase’s original target, new gene mutations are seldom seen at these locations. This approach allows for precise gene correction with minimal off-target mutations. Credit: S. Nakada

“Each chromosome in the genome has a ‘homologous’ copy,” says lead author of the study Akiko Tomita. “Using the NICER technique, heterozygous mutations – in which a mutation appears in one chromosome but not its homologous copy – are repaired using the unmutated homologous chromosome as a template.”

For their initial experiments, the research team used human lymphoblast cells with a known heterozygous mutation in a gene called TK1. When these cells were treated with nickase to induce a single cut in the TK1 region, TK1 activity was recovered at a low rate. However, when the nickase induced multiple nicks in this region on both homologous chromosomes, gene correction efficiency was enhanced approximately seventeen-fold via activation of a cellular repair mechanism.

NICER Gene Editing Method Schematic

Schematic of the NICER gene editing method. A nick is made near the mutation (and is exclusive to the mutant allele). Merely introducing a nick close to the mutation instigates homologous recombination between homologous chromosomes but at a very low rate. However, when one or two additional nicks are made to both alleles, the gene correction efficiency through interhomolog homologous recombination sees a significant boost. Credit: S. Nakada

“Further genomic analysis showed that the NICER technique rarely induced off-target mutations,” says senior author Shinichiro Nakada. “We were also pleased to find that NICER was able to restore the expression of disease-causing genes in cells derived from genetic diseases involving compound heterozygous mutations.”

Because the NICER method does not involve DNA double-strand breaks or the use of exogenous DNA, this technique appears to be a safe alternative to conventional CRISPR/Cas9 methods. NICER may represent a novel approach for the treatment of genetic diseases caused by heterozygous mutations.

Reference: “Inducing multiple nicks promotes interhomolog homologous recombination to correct heterozygous mutations in somatic cells” by Akiko Tomita, Hiroyuki Sasanuma, Tomoo Owa, Yuka Nakazawa, Mayuko Shimada, Takahiro Fukuoka, Tomoo Ogi and Shinichiro Nakada, 15 September 2023, Nature Communications.
DOI: 10.1038/s41467-023-41048-5

>>> Read full article>>>
Copyright for syndicated content belongs to the linked Source : SciTechDaily – https://scitechdaily.com/a-breakthrough-in-genome-editing-how-nicer-outperforms-crispr-cas9/

Tags: BreakthroughGenomescience
Previous Post

Sanwo-Olu to provide health palliatives for residents, subsidise prices of medication

Next Post

A Neuroscience Milestone: Complete Cellular Mapping of a Mammalian Brain

Vibrio pectenicida strain FHCF-3 is a causative agent of sea star wasting disease – Nature

Vibrio pectenicida strain FHCF-3 is a causative agent of sea star wasting disease – Nature

October 23, 2025
Condensation defying gravity – European Space Agency

Condensation defying gravity – European Space Agency

October 23, 2025
Guest educators bring fun environmental science to Kent middle school – MyEasternShoreMD

Guest Educators Ignite Passion for Environmental Science at Kent Middle School

October 23, 2025
Equity Lifestyle Properties, Inc. Profit Rises In Q3, Beats Estimates – Nasdaq

Equity Lifestyle Properties Delivers Impressive Q3 Profit, Exceeding All Expectations

October 23, 2025
Next steps: Technology opens new options for greater mobility – Missouri Independent

Next Steps: How Technology is Opening Exciting New Doors to Greater Mobility

October 23, 2025
American Football Betting Market Report 2025, with Profiles of ESPN, Caesers Entertainment, Entain, Fanatics Sports Betting, Betfred and Other Major and Innovative Companies – Yahoo Finance

American Football Betting Market Report 2025, with Profiles of ESPN, Caesers Entertainment, Entain, Fanatics Sports Betting, Betfred and Other Major and Innovative Companies – Yahoo Finance

October 23, 2025
UIPM 2025 Obstacle World Championships (OCR): All you need to know – Union Internationale de Pentathlon Moderne (UIPM)

Your Ultimate Guide to the 2025 Obstacle World Championships

October 23, 2025
PPPs in Bolivia: The future of our economy? – Dentons

Could PPPs Unlock Bolivia’s Path to Economic Prosperity?

October 23, 2025
Reba McEntire Details Personal Relationship With Late Stepson Brandon Blackstock – KNDU

Reba McEntire Shares Emotional Tribute to Her Late Stepson Brandon Blackstock

October 23, 2025
Mark Peterson receives inaugural APSA Career Achievement Award in Health Politics and Policy – UCLA

Mark Peterson receives inaugural APSA Career Achievement Award in Health Politics and Policy – UCLA

October 23, 2025

Categories

Archives

October 2025
M T W T F S S
 12345
6789101112
13141516171819
20212223242526
2728293031  
« Sep    
Earth-News.info

The Earth News is an independent English-language daily published Website from all around the World News

Browse by Category

  • Business (20,132)
  • Ecology (882)
  • Economy (903)
  • Entertainment (21,774)
  • General (17,757)
  • Health (9,944)
  • Lifestyle (916)
  • News (22,149)
  • People (904)
  • Politics (913)
  • Science (16,114)
  • Sports (21,403)
  • Technology (15,883)
  • World (886)

Recent News

Vibrio pectenicida strain FHCF-3 is a causative agent of sea star wasting disease – Nature

Vibrio pectenicida strain FHCF-3 is a causative agent of sea star wasting disease – Nature

October 23, 2025
Condensation defying gravity – European Space Agency

Condensation defying gravity – European Space Agency

October 23, 2025
  • About
  • Advertise
  • Privacy & Policy
  • Contact

© 2023 earth-news.info

No Result
View All Result

© 2023 earth-news.info

No Result
View All Result

© 2023 earth-news.info

Go to mobile version