Brain imaging turned inside out

Neural connectivity in brain tissue is imaged by labeling the extracellular space.

Since the early days of neuroanatomy, researchers had a choice between two opposed labeling strategies to image neurons and their circuits: label all or label few. In a study in Nature Biotechnology1, Michalska et al. advance a third strategy, achieving a comprehensive view of the nervous system — down to the level of synapses — by labeling the extracellular space in fixed tissue. This makes a new avenue of dense reconstructions of neurons in the brain available to many labs that use an ever growing set of enhanced and super-resolution microscopy techniques.

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Fig. 1: Concept and workflow of CATS.

References

Michalska, J. M. et al. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01911-8 (2023).

Article 
PubMed 

Google Scholar 

Swanson, L. W. & Lichtman, J. W. Annu. Rev. Neurosci.39, 197–216 (2016).

Article 
CAS 
PubMed 

Google Scholar 

Feng, G. et al. Neuron28, 41–51 (2000).

Article 
CAS 
PubMed 

Google Scholar 

Livet, J. et al. Nature450, 56–62 (2007).

Article 
CAS 
PubMed 

Google Scholar 

Wickersham, I. R., Finke, S., Conzelmann, K. K. & Callaway, E. M. Nat. Methods4, 47–49 (2007).

Article 
CAS 
PubMed 

Google Scholar 

Tønnesen, J., Inavalli, V. V. G. K. & Nägerl, U. V. Cell172, 1108–1121.e15 (2018).

Article 
PubMed 

Google Scholar 

Kitamura, K., Judkewitz, B., Kano, M., Denk, W. & Häusser, M. Nat. Methods5, 61–67 (2008).

Article 
CAS 
PubMed 

Google Scholar 

Grassi, D. et al. Cell Rep.42, 112478 (2023).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Pettus, E. H., Christman, C. W., Giebel, M. L. & Povlishock, J. T. J. Neurotrauma11, 507–522 (1994).

Article 
CAS 
PubMed 

Google Scholar 

Williams, P. R. et al. Nat. Commun.5, 5683 (2014).

Article 
CAS 
PubMed 

Google Scholar 

Download references

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Authors and Affiliations

Institute of Neuronal Cell Biology, Technical University of Munich (TUM), Munich, Germany

Shahrzad Askari & Thomas Misgeld

German Center for Neurodegenerative Diseases (DZNE), Munich, Germany

Thomas Misgeld

Munich Cluster for Systems Neurology (SyNergy), Munich, Germany

Thomas Misgeld

Corresponding author

Correspondence to
Thomas Misgeld.

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The authors declare no competing interests.

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Askari, S., Misgeld, T. Brain imaging turned inside out.
Nat Biotechnol (2023). https://doi.org/10.1038/s41587-023-02036-8

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Published: 13 November 2023

DOI: https://doi.org/10.1038/s41587-023-02036-8

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