Electroosmotic flow across nanopores for single-molecule protein sequencing

By using fixed charges to engineer a strong electroosmotic flow, we achieve the unidirectional transport of natural polypeptides across nanopores. Our approach enables native proteins to be transported enzymatically and non-enzymatically in the absence of denaturant and electrophoretic tags, with potential applications for protein sequencing.

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Fig. 1: Polypeptide transport across nanopores.

References

Alfaro, J. A. et al. The emerging landscape of single-molecule protein sequencing technologies. Nat. Methods 18, 604–617 (2021). A review that describes the different approaches to protein sequencing.

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This is a summary of: Sauciuc, A. et al. Translocation of linearized full-length proteins through an engineered nanopore under opposing electrophoretic force. Nat. Biotech. https://doi.org/10.1038/s41587-023-01954-x (2023).