Multiplexed fluorescence imaging enables the visualization of signaling dynamics within a cell by leveraging genetically encoded fluorescent reporters. Previous techniques are limited by the number of signals they can detect within a single cell, or they may require specific hardware that might not be available to all researchers. A recent technique, spatial multiplex imaging, enables the simultaneous imaging of five different reporters within a cell. However, it cannot be used to visualize the organization of proteins in living cells or organelles, or to visualize protein movement in response to a stimulus. In a recent study published in Cell, Qian and colleagues describe temporally multiplexed imaging (TMI), which makes it possible to image multiple reporters using a conventional microscope.
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Nature Biotechnology https://www.nature.com/nbt/
Anahita Bishop
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Bishop, A. Live cell imaging of signaling networks using a conventional microscope.
Nat Biotechnol 42, 31 (2024). https://doi.org/10.1038/s41587-023-02116-9
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Published: 17 January 2024
Issue Date: January 2024
DOI: https://doi.org/10.1038/s41587-023-02116-9
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