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Researchers develop model to study neglected tropical diseases

July 24, 2023
in Science
Researchers develop model to study neglected tropical diseases
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Researchers develop model to study neglected tropical diseases

Credit: Cell Reports Methods (2023). DOI: 10.1016/j.crmeth.2023.100535

Neglected tropical diseases affect more than 1 billion people–one-sixth of the world’s population. Despite their devastating health consequences, these diseases, which occur in some of the world’s poorest and most isolated communities, often receive little attention from global funding agencies and pharmaceutical companies. They can also be difficult for researchers to study, given that many neglected tropical diseases are caused by parasites with large, complex genomes.

Now, in a bid to make it easier to study these neglected diseases, researchers at the George Washington University, in collaboration with colleagues in France and Germany, have developed a model organism from a genetically modified parasitic worm. The team identified four so-called genomic safe harbor sites in the chromosome of the parasitic worm, Schistosoma mansoni. These “Goldilocks” sites are places where genes or genetic elements can be safely inserted without damaging the organism.

The article was published in the journal Cell Reports Methods.

Using CRISPR technology, Wannaporn Ittiprasert Tanno and Paul J. Brindley, research professors of Microbiology, Immunology, and Tropical Medicine at the George Washington University School of Medicine and Health Sciences, successfully inserted a transgene into a genome safe harbor site, that when expressed in the worm, fluoresces as bright green.

The researchers say their method creates a model that could be used for testing additional gene therapies on helminth parasites with the ultimate goal of developing new drugs and vaccines to treat and prevent schistosomiasis and other similar diseases.

More information:
Wannaporn Ittiprasert et al, Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke, Schistosoma mansoni, Cell Reports Methods (2023). DOI: 10.1016/j.crmeth.2023.100535

Citation:
Researchers develop model to study neglected tropical diseases (2023, July 24)
retrieved 24 July 2023
from https://phys.org/news/2023-07-neglected-tropical-diseases.html

This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no
part may be reproduced without the written permission. The content is provided for information purposes only.

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Copyright for syndicated content belongs to the linked Source : Phys.org – https://phys.org/news/2023-07-neglected-tropical-diseases.html

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