GEMS enables mRNA m⁶A methylation sensing in living cells and m⁶A-coupled protein delivery

Thousands of cellular mRNAs contain the methyl modification m6A, which plays critical roles in mRNA processing and gene expression. We developed GEMS, a genetically encoded m6A sensor that provides a simple readout for m6A methylation in living cells and offers a platform for achieving m6A-coupled effector protein expression.

Access options

Access Nature and 54 other Nature Portfolio journals

Get Nature+, our best-value online-access subscription

24,99 € / 30 days

cancel any time

Subscribe to this journal

Receive 12 print issues and online access

209,00 € per year

only 17,42 € per issue

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Log in

Learn about institutional subscriptions

Read our FAQs

Contact customer support

Fig. 1: Schematic of the GEMS system for sensing cellular m6A.

References

Flamand, M. F. et al. The proteins of mRNA modification: writers, readers, and erasers. Annu. Rev. Biochem. 92, 145–173 (2023). This review discusses the function and regulation of m6A and its links to physiology and disease.

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Owens, M. C. et al. Recent technical advances in the study of nucleic acid modifications. Mol. Cell 81, 4116–4136 (2021). This review describes methods for detecting m6A and other RNA modifications.

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Meyer, K. D. DART-seq: an antibody-free method for global m6A detection. Nat. Methods 16, 1275–1280 (2019). This paper describes the DART-seq method for transcriptome-wide m6A mapping.

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Jiang, X. et al. The role of m6A modification in the biological functions and diseases. Signal Transduct. Target. Ther. 6, 74 (2021). This review discusses roles of m6A in physiology and disease.

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Download references

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

This is a summary of: Marayati, B. F., Thompson, M. G., Holley, C. L., Horner, S. M. & Meyer, K. D. Programmable protein expression using a genetically encoded m6A sensor. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01978-3 (2024).

About this article

Cite this article

GEMS enables mRNA m6A methylation sensing in living cells and m6A-coupled protein delivery.
Nat Biotechnol (2024). https://doi.org/10.1038/s41587-023-01997-0

Download citation

Published: 02 January 2024

DOI: https://doi.org/10.1038/s41587-023-01997-0

>>> Read full article>>>
Copyright for syndicated content belongs to the linked Source : Nature.com – https://www.nature.com/articles/s41587-023-01997-0