Monitoring protein proximity with fluorogens

Chemically induced dimerization tools are used for actively perturbing signal transduction in live cells, but most existing methods are not able to be repeatedly reversed. In a study published in Nature Methods, Bottone et al. introduce a system called CATCHFIRE (chemically assisted tethering of chimera by fluorogenic-induced recognition), which allows the monitoring of reversibly induced protein proximity. This method relies on the chemical induction of dimerization using a fluorogen, which results in the assembled complex fluorescing, enabling real-time visualization of interactions with precise temporal control.

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Despang, A. Monitoring protein proximity with fluorogens.
Nat Biotechnol 41, 1386 (2023). https://doi.org/10.1038/s41587-023-02000-6

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Published: 12 October 2023

Issue Date: October 2023

DOI: https://doi.org/10.1038/s41587-023-02000-6

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