Chemically induced dimerization tools are used for actively perturbing signal transduction in live cells, but most existing methods are not able to be repeatedly reversed. In a study published in Nature Methods, Bottone et al. introduce a system called CATCHFIRE (chemically assisted tethering of chimera by fluorogenic-induced recognition), which allows the monitoring of reversibly induced protein proximity. This method relies on the chemical induction of dimerization using a fluorogen, which results in the assembled complex fluorescing, enabling real-time visualization of interactions with precise temporal control.
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Despang, A. Monitoring protein proximity with fluorogens.
Nat Biotechnol 41, 1386 (2023). https://doi.org/10.1038/s41587-023-02000-6
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Published: 12 October 2023
Issue Date: October 2023
DOI: https://doi.org/10.1038/s41587-023-02000-6
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