Precise and versatile genome editing with click editors

Precise and versatile genome editing with click editors

Research Briefing

Published: 22 July 2024

Nature Biotechnology

(2024)Cite this article

We developed click editors, comprising HUH endonucleases, DNA-dependent DNA polymerases and CRISPR–Cas9 nickases, which together enable programmable precision genome engineering from simple DNA templates.

This is a preview of subscription content, access via your institution

Access options

Access Nature and 54 other Nature Portfolio journals

Get Nature+, our best-value online-access subscription

24,99 € / 30 days

cancel any time

Subscribe to this journal

Receive 12 print issues and online access

209,00 € per year

only 17,42 € per issue

Buy this article

Purchase on Springer LinkInstant access to full article PDF

Prices may be subject to local taxes which are calculated during checkout

Additional access options:

Log in

Learn about institutional subscriptions

Read our FAQs

Contact customer support

Fig. 1: Click editing enzymes and mechanism.

References

Halperin, S. O. et al. CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window. Nature550, 248–252 (2018). This manuscript describes the fusion of an error-prone polymerase to CRISPR–Cas9 to mutagenize specified genomic regions.

Article 

Google Scholar 

Anzalone, A. V. et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature576, 149–157 (2019). This paper reports the development of prime editing, a precise genome-editing approach that uses reverse transcriptases to write specific edits into a genome.

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Levesque, S., Cosentino, A., Verma, A., Genovese, P. & Bauer, D. E. Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02266-4 (2024). This manuscript reports the dependence of RT-based genome editing technologies, such as prime editors, on cellular dNTP levels.

Aird, E. L. et al. Increasing Cas9-mediated homology-directed repair efficiency through covalent tethering of DNA repair template. Commun. Biol.1, 54 (2018). This study describes the use of HUHes to recruit ssDNA templates to Cas9-mediated DNA breaks to improve homology-directed repair efficiencies.

Article 
PubMed 
PubMed Central 

Google Scholar 

Liu, B. et al. Targeted genome editing with a DNA-dependent DNA polymerase and exogenous DNA-containing templates. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01947-w (2023). This paper reports the development of DNA polymerase-based editors using RNA or DNA substrates for precise genome editing.

Article 
PubMed 
PubMed Central 

Google Scholar 

Download references

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

This is a summary of: Ferreira da Silva, J. et al. Click editing enables programmable genome writing using DNA polymerases and HUH endonucleases. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02324-x (2024).

About this article

Cite this article

Precise and versatile genome editing with click editors.
Nat Biotechnol (2024). https://doi.org/10.1038/s41587-024-02340-x

Download citation

Published: 22 July 2024

DOI: https://doi.org/10.1038/s41587-024-02340-x

>>> Read full article>>>
Copyright for syndicated content belongs to the linked Source : Nature.com – https://www.nature.com/articles/s41587-024-02340-x

Exit mobile version