* . *
  • About
  • Advertise
  • Privacy & Policy
  • Contact
Saturday, August 23, 2025
Earth-News
  • Home
  • Business
  • Entertainment
    From polka to Poison, Corn Palace adjusts entertainment offerings with the times – Mitchell Republic

    From polka to Poison, Corn Palace adjusts entertainment offerings with the times – Mitchell Republic

    How to watch ‘F1: The Movie’ on Prime Video – About Amazon

    Experience the Thrill: How to Stream ‘F1: The Movie’ on Prime Video

    FOX One is now available on Prime Video: Here’s everything to know – About Amazon

    FOX One is now available on Prime Video: Here’s everything to know – About Amazon

    What Are Our Predictions for Taylor Swift’s ‘Life of a Showgirl’ Based on What She’s Told Us So Far? – yahoo.com

    Uncover the Hidden Surprises in Taylor Swift’s ‘Life of a Showgirl’ – Can You Decode Her Clues?

    Bama Dining Serving Up New Food, Entertainment – UA News Center

    Bama Dining Launches Thrilling New Food and Entertainment Experiences

    The latest on Williams-Brice Stadium renovations, entertainment district – On3

    Discover the Thrilling New Upgrades Coming to Williams-Brice Stadium and Its Vibrant Entertainment District!

  • General
  • Health
  • News

    Cracking the Code: Why China’s Economic Challenges Aren’t Shaking Markets, Unlike America’s” – Bloomberg

    Trump’s Narrow Window to Spread the Truth About Harris

    Trump’s Narrow Window to Spread the Truth About Harris

    Israel-Gaza war live updates: Hamas leader Ismail Haniyeh assassinated in Iran, group says

    Israel-Gaza war live updates: Hamas leader Ismail Haniyeh assassinated in Iran, group says

    PAP Boss to Niger Delta Youths, Stay Away from the Protest

    PAP Boss to Niger Delta Youths, Stay Away from the Protest

    Court Restricts Protests In Lagos To Freedom, Peace Park

    Court Restricts Protests In Lagos To Freedom, Peace Park

    Fans React to Jazz Jennings’ Inspiring Weight Loss Journey

    Fans React to Jazz Jennings’ Inspiring Weight Loss Journey

    Trending Tags

    • Trump Inauguration
    • United Stated
    • White House
    • Market Stories
    • Election Results
  • Science
  • Sports
  • Technology
    Alcorn State awarded grant to boost STEM with VR technology – WJTV

    Alcorn State Secures Grant to Transform STEM Education Through Cutting-Edge VR Technology

    Hyundai: The Only Way To Beat China Is To Embrace Technology – InsideEVs

    Hyundai’s Bold Strategy to Outpace China with Cutting-Edge Technology

    Teaching older adults how to use technology – WWNY

    Empowering Older Adults to Master Technology with Confidence

    Denver man receives pacemaker using new technology – CBS News

    Denver Man’s Life Transformed by Revolutionary Pacemaker Technology

    Morgan County Rescue Squad is using new technology to recover people missing in the water faster – WAFF

    Morgan County Rescue Squad is using new technology to recover people missing in the water faster – WAFF

    YSU to celebrate launch of new radiology technology program – WKBN.com

    YSU Launches Exciting New Radiology Technology Program Celebration

    Trending Tags

    • Nintendo Switch
    • CES 2017
    • Playstation 4 Pro
    • Mark Zuckerberg
No Result
View All Result
  • Home
  • Business
  • Entertainment
    From polka to Poison, Corn Palace adjusts entertainment offerings with the times – Mitchell Republic

    From polka to Poison, Corn Palace adjusts entertainment offerings with the times – Mitchell Republic

    How to watch ‘F1: The Movie’ on Prime Video – About Amazon

    Experience the Thrill: How to Stream ‘F1: The Movie’ on Prime Video

    FOX One is now available on Prime Video: Here’s everything to know – About Amazon

    FOX One is now available on Prime Video: Here’s everything to know – About Amazon

    What Are Our Predictions for Taylor Swift’s ‘Life of a Showgirl’ Based on What She’s Told Us So Far? – yahoo.com

    Uncover the Hidden Surprises in Taylor Swift’s ‘Life of a Showgirl’ – Can You Decode Her Clues?

    Bama Dining Serving Up New Food, Entertainment – UA News Center

    Bama Dining Launches Thrilling New Food and Entertainment Experiences

    The latest on Williams-Brice Stadium renovations, entertainment district – On3

    Discover the Thrilling New Upgrades Coming to Williams-Brice Stadium and Its Vibrant Entertainment District!

  • General
  • Health
  • News

    Cracking the Code: Why China’s Economic Challenges Aren’t Shaking Markets, Unlike America’s” – Bloomberg

    Trump’s Narrow Window to Spread the Truth About Harris

    Trump’s Narrow Window to Spread the Truth About Harris

    Israel-Gaza war live updates: Hamas leader Ismail Haniyeh assassinated in Iran, group says

    Israel-Gaza war live updates: Hamas leader Ismail Haniyeh assassinated in Iran, group says

    PAP Boss to Niger Delta Youths, Stay Away from the Protest

    PAP Boss to Niger Delta Youths, Stay Away from the Protest

    Court Restricts Protests In Lagos To Freedom, Peace Park

    Court Restricts Protests In Lagos To Freedom, Peace Park

    Fans React to Jazz Jennings’ Inspiring Weight Loss Journey

    Fans React to Jazz Jennings’ Inspiring Weight Loss Journey

    Trending Tags

    • Trump Inauguration
    • United Stated
    • White House
    • Market Stories
    • Election Results
  • Science
  • Sports
  • Technology
    Alcorn State awarded grant to boost STEM with VR technology – WJTV

    Alcorn State Secures Grant to Transform STEM Education Through Cutting-Edge VR Technology

    Hyundai: The Only Way To Beat China Is To Embrace Technology – InsideEVs

    Hyundai’s Bold Strategy to Outpace China with Cutting-Edge Technology

    Teaching older adults how to use technology – WWNY

    Empowering Older Adults to Master Technology with Confidence

    Denver man receives pacemaker using new technology – CBS News

    Denver Man’s Life Transformed by Revolutionary Pacemaker Technology

    Morgan County Rescue Squad is using new technology to recover people missing in the water faster – WAFF

    Morgan County Rescue Squad is using new technology to recover people missing in the water faster – WAFF

    YSU to celebrate launch of new radiology technology program – WKBN.com

    YSU Launches Exciting New Radiology Technology Program Celebration

    Trending Tags

    • Nintendo Switch
    • CES 2017
    • Playstation 4 Pro
    • Mark Zuckerberg
No Result
View All Result
Earth-News
No Result
View All Result
Home Science

Translocation of linearized full-length proteins through an engineered nanopore under opposing electrophoretic force

September 19, 2023
in Science
Translocation of linearized full-length proteins through an engineered nanopore under opposing electrophoretic force
Share on FacebookShare on Twitter

Gu, L.-Q., Cheley, S. & Bayley, H. Electroosmotic enhancement of the binding of a neutral molecule to a transmembrane pore. Proc. Natl. Acad. Sci. USA 100, 15498–15503 (2003).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Huang, G., Willems, K., Soskine, M., Wloka, C. & Maglia, G. Electro-osmotic capture and ionic discrimination of peptide and protein biomarkers with FraC nanopores. Nat. Commun. 8, 935 (2017).

Article 
PubMed 
PubMed Central 

Google Scholar 

Huang, G. et al. Electro-osmotic vortices promote the capture of folded proteins by PlyAB nanopores. Nano Lett. 20, 3819–3827 (2020).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Asandei, A. et al. Electroosmotic trap against the electrophoretic force near a protein nanopore reveals peptide dynamics during capture and translocation. ACS Appl. Mater. Interfaces 8, 13166–13179 (2016).

Article 
CAS 
PubMed 

Google Scholar 

Willems, K. et al. Engineering and modeling the electrophoretic trapping of a single protein inside a nanopore. ACS Nano 13, 9980–9992 (2019).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Gubbiotti, A. et al. Electroosmosis in nanopores: computational methods and technological applications. Adv. Phys. X 7, 2036638 (2022).

Google Scholar 

Brinkerhoff, H., Kang, A. S. W., Liu, J., Aksimentiev, A. & Dekker, C. Multiple rereads of single proteins at single–amino acid resolution using nanopores. Science 374, 1509–1513 (2021).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Yan, S. et al. Single molecule ratcheting motion of peptides in a Mycobacterium smegmatis porin A (MspA) nanopore. Nano Lett. 21, 6703–6710 (2021).

Article 
CAS 
PubMed 

Google Scholar 

Chen, Z. et al. Controlled movement of ssDNA conjugated peptide through Mycobacterium smegmatis porin A (MspA) pore by a helicase motor for peptide sequencing application. Chem. Sci. 12, 15750–15756 (2021).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Rosen, C. B., Rodriguez-Larrea, D. & Bayley, H. Single-molecule site-specific detection of protein phosphorylation with a nanopore. Nat. Biotechnol. 32, 179–181 (2014).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Yu, L. et al. Unidirectional single-file transport of full-length proteins through a nanopore. Nat. Biotechnol. 41,1130–1139 (2023).

Article 
CAS 
PubMed 

Google Scholar 

Biesemans, A., Soskine, M. & Maglia, G. A protein rotaxane controls the translocation of proteins across a ClyA nanopore. Nano Lett. 15, 6076–6081 (2015).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Aksimentiev, A. & Schulten, K. Imaging α-hemolysin with molecular dynamics: ionic conductance, osmotic permeability, and the electrostatic potential map. Biophys. J. 88, 3745–3761 (2005).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Bonome, E. L., Cecconi, F. & Chinappi, M. Electroosmotic flow through an α-hemolysin nanopore. Microfluid. Nanofluid. 21,96 (2017).

Article 

Google Scholar 

Bétermier, F. et al. Single-sulfur atom discrimination of polysulfides with a protein nanopore for improved batteries. Commun. Mater. 1, 59 (2020).

Article 

Google Scholar 

Di Muccio, G., Morozzo della Rocca, B. & Chinappi, M. Geometrically induced selectivity and unidirectional electroosmosis in uncharged nanopores. ACS Nano 16, 8716–8728 (2022).

Article 
PubMed 
PubMed Central 

Google Scholar 

Raffy, S., Sassoon, N., Hofnung, M. & Betton, J.-M. Tertiary structure-dependence of misfolding substitutions in loops of the maltose-binding protein. Protein Sci. 7, 2136–2142 (1998).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Fonin, A. V. et al. Spectral characteristics of the mutant form GGBP/H152C of D-glucose/D-galactose-binding protein labeled with fluorescent dye BADAN: influence of external factors. PeerJ 2, e275 (2014).

Article 
PubMed 
PubMed Central 

Google Scholar 

Ohmae, E., Sasaki, Y. & Gekko, K. Effects of five-tryptophan mutations on structure, stability and function of Escherichia coli dihydrofolate reductase. J. Biochem. 130, 439–447 (2001).

Article 
CAS 
PubMed 

Google Scholar 

Japrung, D., Henricus, M., Li, Q. H., Maglia, G. & Bayley, H. Urea facilitates the translocation of single-stranded DNA and RNA through the α-hemolysin nanopore. Biophys. J. 98, 1856–1863 (2010).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Pastoriza-Gallego, M. et al. Evidence of unfolded protein translocation through a protein nanopore. ACS Nano 8, 11350–11360 (2014).

Article 
CAS 
PubMed 

Google Scholar 

Oukhaled, G. et al. Unfolding of proteins and long transient conformations detected by single nanopore recording. Phys. Rev. Lett. 98, 158101 (2007).

Pastoriza-Gallego, M. et al. Dynamics of unfolded protein transport through an aerolysin pore. J. Am. Chem. Soc. 133, 2923–2931 (2011).

Article 
CAS 
PubMed 

Google Scholar 

Rodriguez-Larrea, D. & Bayley, H. Multistep protein unfolding during nanopore translocation. Nat. Nanotechnol. 8, 288–295 (2013).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Humphrey, W., Dalke, A. & Schulten, K. VMD: visual molecular dynamics. J. Mol. Graph. 14, 33–38 (1996).

Article 
CAS 
PubMed 

Google Scholar 

Bitinaite, J. et al. USER friendly DNA engineering and cloning method by uracil excision. Nucleic Acids Res. 35, 1992–2002 (2007).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Cavaleiro, A. M., Kim, S. H., Seppälä, S., Nielsen, M. T. & Nørholm, M. H. H. Accurate DNA assembly and genome engineering with optimized uracil excision cloning. ACS Synth. Biol. 4, 1042–1046 (2015).

Article 
CAS 
PubMed 

Google Scholar 

Nørholm, M. H. A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering. BMC Biotechnol. 10, 21 (2010).

Article 
PubMed 
PubMed Central 

Google Scholar 

Zhang, S. et al. Bottom-up fabrication of a proteasome–nanopore that unravels and processes single proteins. Nat. Chem. 13, 1192–1199 (2021).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Maglia, G., Heron, A. J. J. A. J., Stoddart, D., Japrung, D. & Bayley, H. Analysis of single nucleic acid molecules with protein nanopores. Methods Enzymol. 475, 591–623 (2010).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Waterhouse, A. et al. SWISS-MODEL: homology modelling of protein structures and complexes. Nucleic Acids Res. 46, W296–W303 (2018).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Tanaka, Y. et al. 2-Methyl-2,4-pentanediol induces spontaneous assembly of staphylococcal α-hemolysin into heptameric pore structure. Protein Sci. 20, 448–456 (2011).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Lambey, P. et al. Structural insights into recognition of chemokine receptors by Staphylococcus aureus leukotoxins. eLife 11, e72555 (2022).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Nocadello, S. et al. Crystal structures of the components of the Staphylococcus aureus leukotoxin ED. Acta Crystallogr. Sect. D Struct. Biol. 72, 113–120 (2016).

Article 
CAS 

Google Scholar 

Jumper, J. et al. Highly accurate protein structure prediction with AlphaFold. Nature 596, 583–589 (2021).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Pettersen, E. F. UCSF Chimera—a visualization system for exploratory research and analysis. J. Comput. Chem. 25, 1605–12 (2004).

Article 
CAS 
PubMed 

Google Scholar 

Phillips, J. C. et al. Scalable molecular dynamics with NAMD. J. Comput. Chem. 26, 1781–1802 (2005).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

Best, R. B. et al. Optimization of the additive CHARMM all-atom protein force field targeting improved sampling of the backbone φ, ψ and side-chain χ(1) and χ(2) dihedral angles. J. Chem. Theory Comput. 8, 3257–3273 (2012).

Jorgensen, W. L., Chandrasekhar, J., Madura, J. D., Impey, R. W. & Klein, M. L. Comparison of simple potential functions for simulating liquid water. J. Chem. Phys. 79, 926–935 (1983).

Article 
CAS 

Google Scholar 

Yoo, J. & Aksimentiev, A. Improved parametrization of Li+, Na+, K+, and Mg2+ Ions for all-atom molecular dynamics simulations of nucleic acid systems. J. Phys. Chem. Lett. 3, 45–50 (2012).

Article 
CAS 

Google Scholar 

Essmann, U. et al. A smooth particle mesh Ewald method. J. Chem. Phys. 103, 8577–8593 (1995).

Article 
CAS 

Google Scholar 

Miyamoto, S. & Kollman, P. A. Settle: an analytical version of the SHAKE and RATTLE algorithm for rigid water models. J. Comput. Chem. 13, 952–962 (1992).

Article 
CAS 

Google Scholar 

Andersen, H. C. Rattle: a ‘velocity’ version of the shake algorithm for molecular dynamics calculations. J. Comput. Phys. 52, 24–34 (1983).

Article 
CAS 

Google Scholar 

Martyna, G. J., Tobias, D. J. & Klein, M. L. Constant pressure molecular dynamics algorithms. J. Chem. Phys. 101, 4177–4189 (1994).

Article 
CAS 

Google Scholar 

Gumbart, J., Khalili-Araghi, F., Sotomayor, M. & Roux, B. Constant electric field simulations of the membrane potential illustrated with simple systems. Biochim. Biophys. Acta 1818, 294–302 (2012).

Article 
CAS 
PubMed 

Google Scholar 

Crozier, P. S., Henderson, D., Rowley, R. L. & Busath, D. D. Model channel ion currents in NaCl-extended simple point charge water solution with applied-field molecular dynamics. Biophys. J. 81, 3077–3089 (2001).

Article 
CAS 
PubMed 
PubMed Central 

Google Scholar 

>>> Read full article>>>
Copyright for syndicated content belongs to the linked Source : Nature.com – https://www.nature.com/articles/s41587-023-01954-x

Tags: linearizedscienceTranslocation
Previous Post

Electroosmotic flow across nanopores for single-molecule protein sequencing

Next Post

The great outdoors: China’s appetite for adventure sports booms

Putin wants to attend 2026 FIFA World Cup in the U.S. “very badly,” Trump says – Axios

Putin Eager to Attend 2026 FIFA World Cup in the U.S., Says Trump

August 23, 2025
How the semiconductor boom and ASU are transforming Arizona’s economy – ASU News

How the semiconductor boom and ASU are transforming Arizona’s economy – ASU News

August 23, 2025
From polka to Poison, Corn Palace adjusts entertainment offerings with the times – Mitchell Republic

From polka to Poison, Corn Palace adjusts entertainment offerings with the times – Mitchell Republic

August 23, 2025
Luther College to host discussion exploring links between soil and food health – Luther College

Luther College to host discussion exploring links between soil and food health – Luther College

August 23, 2025
OPINION | WOODY BASSETT: Can the nation do better in its politics? Can the Democrats? – Northwest Arkansas Democrat-Gazette

OPINION | WOODY BASSETT: Can the nation do better in its politics? Can the Democrats? – Northwest Arkansas Democrat-Gazette

August 23, 2025
Crossings: How Road Ecology is Shaping the Future of Our Planet – Living on Earth

Crossings: How Road Ecology is Shaping the Future of Our Planet

August 23, 2025
Toyota Friday Night Rivals kicks off season with Elizabethton at Science Hill – WCYB

Toyota Friday Night Rivals Ignite the Season with Elizabethton vs. Science Hill Showdown

August 23, 2025
Star Trek as Refuge for the Science-Minded – The Provincetown Independent

How Star Trek Ignites Passion and Empowers the Science-Minded

August 23, 2025
Refresh your wardrobe at these 11 QTS award-winning fashion and lifestyle stores – Time Out Worldwide

Transform Your Look with These 11 Must-Visit Fashion and Lifestyle Stores

August 23, 2025
Alcorn State awarded grant to boost STEM with VR technology – WJTV

Alcorn State Secures Grant to Transform STEM Education Through Cutting-Edge VR Technology

August 23, 2025

Categories

Archives

August 2025
MTWTFSS
 123
45678910
11121314151617
18192021222324
25262728293031
« Jul    
Earth-News.info

The Earth News is an independent English-language daily published Website from all around the World News

Browse by Category

  • Business (20,132)
  • Ecology (785)
  • Economy (806)
  • Entertainment (21,685)
  • General (16,624)
  • Health (9,846)
  • Lifestyle (818)
  • News (22,149)
  • People (807)
  • Politics (815)
  • Science (16,018)
  • Sports (21,304)
  • Technology (15,786)
  • World (787)

Recent News

Putin wants to attend 2026 FIFA World Cup in the U.S. “very badly,” Trump says – Axios

Putin Eager to Attend 2026 FIFA World Cup in the U.S., Says Trump

August 23, 2025
How the semiconductor boom and ASU are transforming Arizona’s economy – ASU News

How the semiconductor boom and ASU are transforming Arizona’s economy – ASU News

August 23, 2025
  • About
  • Advertise
  • Privacy & Policy
  • Contact

© 2023 earth-news.info

No Result
View All Result

© 2023 earth-news.info

No Result
View All Result

© 2023 earth-news.info

Go to mobile version